Dystroglycan depletion inhibits the functions of differentiated HL-60 cells |
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| Authors: | Alma Delia Martí nez-Zá rate,Ivette Martí nez-Vieyra,Lea Alonso-Rangel,Bulmaro Cisneros,Steve J. Winder,Doris Cerecedo |
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| Affiliation: | 1. Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico;2. Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav-IPN), Mexico City, Mexico;3. Department of Biomedical Science, University of Sheffield, Sheffield, UK |
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| Abstract: | Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to neutrophils. Depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and expression of markers of differentiation. These findings strongly implicate dystroglycan as a key membrane adhesion protein involved in the differentiation process in HL-60 cells. |
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| Keywords: | Dystroglycan HL-60 cells Differentiation Phagocytosis Respiratory burst activity Migration |
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