Selective stimulatory action of olfactory ensheathing glia-conditioned medium on oligodendroglial differentiation,with additional reference to signaling mechanisms |
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| Authors: | Litia A Carvalho Louise C Vitorino Roberta PM Guimarães Silvana Allodi Ricardo A de Melo Reis Leny A Cavalcante |
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| Institution: | 1. Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, Ilha do Fundão, 21949-902 Rio de Janeiro, Brazil;2. Programa de Pós-Graduação em Ciências Biológicas (Fisiologia), Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, Ilha do Fundão, 21949-902 Rio de Janeiro, Brazil;3. Programa de Pós-Graduação em Ciências Biológicas (Biofísica), Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, Ilha do Fundão, 21949-902 Rio de Janeiro, Brazil |
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| Abstract: | We examined the effects of conditioned medium from olfactory ensheathing glia (OEGCM) on the differentiation of oligodendrocytes in mixed cultures of early postnatal hippocampi. Differentiation was judged from the numerical density (ND) of cells immunoreactive to 2′3′ cyclic nucleotide 3′phosphodiesterase (CNPase) and O4 antibodies. NDs increased according to inverted-U dose–response curves, particularly for CNPase+ cells (9-fold at optimal dilution) and these changes were blocked by inhibitors of ERK1, p38-MAPK, and PI3K. Our results raise the possibility that OEG secreted factor(s) may counteract demyelination induced by trauma, neurodegenerative diseases, and advanced age, and should stimulate novel methods to deliver these factors and/or potentiating chemicals. |
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| Keywords: | Oligodendrocytes Hippocampus ERK1 PI3K p38-MAPK |
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