Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model |
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Authors: | Yongde Xu Hongen Lei Ruili Guan Zhezhu Gao Huixi Li Lin Wang Weidong Song Bing Gao Zhongcheng Xin |
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Institution: | 1. Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China;2. Department of Urology, Peking University First Hospital and the Institute of Urology, Peking University, Beijing 100034, China |
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Abstract: | Streptozotocin (STZ) induced diabetic model has been widely used to study the effects of diabetes mellitus (DM) on male infertility, but it remains unclear whether the responses in this model are due to hyperglycemia or STZ per se. This study was designed to investigate the mechanism of STZ on testicular dysfunction. In the present study, sperm characteristics, serum testosterone, steroidogenic enzymes (StAR and 3β-HSD), and the vimentin apical extension of sertoli cells decreased significantly in the STZ group compared with those in the normal controls (p < 0.05), while Johnsen’s score, testicular lipid peroxidation, spermatogenic cell apoptosis, and the expressions of NF-κB and Wnt4 significantly increased (p < 0.05). Insulin replacement mainly restored the decreased serum testosterone and steroidogenic enzymes, but not other parameters. The results indicated that spermatogenic dysfunction in the early stage of STZ-induced diabetic rats was due to direct STZ cytotoxicity to sertoli cells, which could be regulated by Wnt4 and NF-κB, while steroidogenic dysfunction might be a direct or indirect consequence of insulin deficiency. The results suggested that STZ-induced diabetic model, at least in the early stage, is not suitable to study the diabetes-related spermatogenic dysfunction. |
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Keywords: | Control the Control group STZ streptozotocin or the STZ group STZ + In the Insulin group (STZ induced diabetic rats treated with insulin) DM diabetes mellitus WB western blotting MDA malondialdehyde TUNEL terminal transferase-mediated dUTP-biotin nick end-labeling NF-κB nuclear factor-κB |
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