Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system |
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Authors: | Adam H Kramer Julia Joos-Vandewalle Adrienne L Edkins Carminita L Frost Earl Prinsloo |
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Affiliation: | 1. Biomedical Biotechnology Research Unit, Department of Biochemistry, Microbiology and Biotechnology, PO Box 94, Rhodes University, Grahamstown 6140, South Africa;2. Department of Biochemistry and Microbiology, PO Box 77000, Nelson Mandela Metropolitan University, Port Elizabeth 6031, South Africa |
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Abstract: | Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors. |
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Keywords: | INS insulin DEX dexamethasone IBMX 3-isobutyl-1-methylxanthine RTCA Real-time Cell Analyser ROSI rosiglitazone |
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