Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells |
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| Authors: | Helen Pullisaar Janne E Reseland Håvard J Haugen Jan E Brinchmann Esben Østrup |
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| Institution: | 1. Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Norway;2. Institute of Immunology, Oslo University Hospital Rikshospitalet, Norway;3. Norwegian Center for Stem Cell Research, Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Norway |
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| Abstract: | Bone tissue engineering requires an osteoconductive scaffold, multipotent cells with regenerative capacity and bioactive molecules. In this study we investigated the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) on titanium dioxide (TiO2) scaffold coated with alginate hydrogel containing various concentrations of simvastatin (SIM). The mRNA expression of osteoblast-related genes such as collagen type I alpha 1 (COL1A1), alkaline phosphatase (ALPL), osteopontin (SPP1), osteocalcin (BGLAP) and vascular endothelial growth factor A (VEGFA) was enhanced in hAD-MSCs cultured on scaffolds with SIM in comparison to scaffolds without SIM. Furthermore, the secretion of osteoprotegerin (OPG), vascular endothelial growth factor A (VEGFA), osteopontin (OPN) and osteocalcin (OC) to the cell culture medium was higher from hAD-MSCs cultured on scaffolds with SIM compared to scaffolds without SIM. The TiO2 scaffold coated with alginate hydrogel containing SIM promote osteogenic differentiation of hAD-MSCs in vitro, and demonstrate feasibility as scaffold for hAD-MSC based bone tissue engineering. |
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| Keywords: | hAD-MSCs human adipose tissue-derived mesenchymal stem cells TiO2 titanium dioxide SIM simvastatin OPN osteopontin protein SPP1 osteopontin mRNA OPG osteoprotegerin protein TNFRSF11B osteoprotegerin mRNA OC osteocalcin protein BGLAP osteocalcin mRNA VEGFA vascular endothelial growth factor A protein VEGFA vascular endothelial growth factor A mRNA LDH lactate dehydrogenase ALP alkaline phosphatase protein ALPL alkaline phosphatase mRNA qRT-PCR quantitative RT-PCR |
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