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Salvianolic acid B protects cardiomyocytes from angiotensin II-induced hypertrophy via inhibition of PARP-1
Authors:Min Liu  Jiantao Ye  Si Gao  Wei Fang  Hong Li  Biao Geng  Jian Zou  Xi Chen  Shaorui Chen  Luankun Zhang  Zhongbao Yue  Yunzi Ma  Hui Gao  Zhuoming Li  Peiqing Liu
Affiliation:1. Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China;2. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China
Abstract:Salvianolic acid B (SalB), one of the major bioactive components in Salviamiltiorrhiza, has plenty of cardioprotective effects. The present study was designed to investigate the effect of SalB on angiotensin II (AngII)-induced hypertrophy in neonatal rat cardiomyocytes, and to find out whether or not this effect is attributed to inhibition of poly (ADP-ribose) polymerase-1 (PARP-1), which plays a key role in cardiac hypertrophy. Our results showed that SalB prevented the cardiomyocytes from AngII-induced hypertrophy, associated with attenuation of the mRNA expressions of atrial natriuretic factor and brain natriuretic peptide, and reduction in the cell surface area. SalB inhibited the activity of PARP-1. The inhibitory effect was comparable to that of the PARP-1 inhibitor 3-Aminobenzamide (3-AB). In addition, SalB reversed the depletion of cellular NAD+ induced by AngII. Moreover, overexpression of PARP-1 attenuated the anti-hypertrophic effect of SalB. These observations suggested that SalB prevented the cardiomyocytes from AngII-induced hypertrophy, at least partially through inhibition of PARP-1. Moreover, SalB attenuated the generation of oxidative stress via suppression of NADPH oxidase 2 and 4, which might probably contribute to the inhibition of PARP-1. These present findings may shed new light on the understanding of the cardioprotective effect of SalB.
Keywords:SalB, Salvianolic acid B   3-AB, 3-Aminobenzamide   ANF, atrial natriuretic factor   AngII, Angiotensin II   BNP, brain natriuretic polypeptide   DHE, dihydroethidium   MTT, 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide   NAD, nicotinamide adenine dinucleotide   NOX, NADPH oxidase   PARP-1, poly (ADP-ribose) polymerase-1   ROS, reactive oxygen species
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