X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC) |
| |
Authors: | J Domingo Meza-Aguilar Petra Fromme Alfredo Torres-Larios Guillermo Mendoza-Hernández Ulises Hernandez-Chiñas Roberto A Arreguin-Espinosa de los Monteros Carlos A Eslava Campos Raimund Fromme |
| |
Institution: | 1. Departamento de Salud Pública Facultad de Medicina UNAM, Ciudad Universitaria Coyoacán 04510, D.F., Mexico;2. Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato Oncología e Investigación, Hospital Infantil de México Federico Gómez 06720, D.F., Mexico;3. Department of Chemistry and Biochemistry, Arizona State University, Physical Sciences BLDG D-102, Tempe, AZ 85287, USA;4. Instituto de Fisiología Celular UNAM, Ciudad Universitaria Coyoacán 04510, D.F., Mexico;5. Instituto de Química UNAM, Ciudad Universitaria Coyoacán 04510, D.F, Mexico |
| |
Abstract: | Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100 kDa protein at 2.3 Å resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces loss of actin stress fibers. While Pet (pdb code: 4OM9) shows only a sequence identity of 50% compared to the closest related protein sequence, extracellular serine protease plasmid (EspP) the structural features of both proteins are conserved. A closer structural look reveals that Pet contains a β-pleaded sheet at the sequence region of residues 181–190, the corresponding structural domain in EspP consists of a coiled loop. Secondary, the Pet passenger domain features a more pronounced beta sheet between residues 135 and 143 compared to the structure of EspP. |
| |
Keywords: | Plasmid encoded toxin(Pet) Passenger domain Autotransporter 4OM9 EAEC SPATE |
本文献已被 ScienceDirect 等数据库收录! |
|