Anthraquinones quinizarin and danthron unwind negatively supercoiled DNA and lengthen linear DNA |
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| Authors: | Valé ria Verebová ,Jozef Adamcik,Patrik Danko,Du&scaron an Podhradský ,Pavol Mi&scaron kovský ,Jana Staničová |
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| Affiliation: | 1. Institute of Biophysics, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia;2. Food and Soft Materials Science, Institute of Food, Nutrition & Health, ETH Zurich, Schmelzbergstrasse 9, CH-8092 Zürich, Switzerland;3. Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P.J. Šafárik University, Moyzesova 11, 041 54 Košice, Slovakia;4. Department of Biophysics, Faculty of Sciences, P.J. Šafárik University, Jesenná 5, 041 54 Košice, Slovakia;5. Center for Interdisciplinary Biosciences, Faculty of Sciences, P.J. Šafárik University, Jesenná 5, 041 54 Košice, Slovakia |
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| Abstract: | The intercalating drugs possess a planar aromatic chromophore unit by which they insert between DNA bases causing the distortion of classical B-DNA form. The planar tricyclic structure of anthraquinones belongs to the group of chromophore units and enables anthraquinones to bind to DNA by intercalating mode. The interactions of simple derivatives of anthraquinone, quinizarin (1,4-dihydroxyanthraquinone) and danthron (1,8-dihydroxyanthraquinone), with negatively supercoiled and linear DNA were investigated using a combination of the electrophoretic methods, fluorescence spectrophotometry and single molecule technique an atomic force microscopy. The detection of the topological change of negatively supercoiled plasmid DNA, unwinding of negatively supercoiled DNA, corresponding to appearance of DNA topoisomers with the low superhelicity and an increase of the contour length of linear DNA in the presence of quinizarin and danthron indicate the binding of both anthraquinones to DNA by intercalating mode. |
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| Keywords: | AGE, agarose gel electrophoresis TGGE, temperature gradient gel electrophoresis AFM, atomic force microscopy DMSO, dimethyl sulfoxide EDTA, ethylenediaminetetraacetic acid APTES, 3-aminopropyltriethoxysilane Tris, tris(hydroxymethyl)aminomethane TBE, tris&ndash borate&ndash EDTA EtBr, ethidium bromide |
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