Inhibitory role of Id1 on TGF-β-induced collagen expression in human dermal fibroblasts |
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Authors: | Young-Jin Je Dae-Kyoung ChoiKyung-Cheol Sohn Hyeong-Rae KimMyung Im Young LeeJeung-Hoon Lee Chang-Deok Kim Young-Joon Seo |
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Institution: | Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Republic of Korea |
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Abstract: | Inhibitor of DNA binding 1 (Id1) is a basic helix-loop-helix (bHLH) protein that has a variety of functional roles in cellular events including differentiation, cell cycle and cancer development. In addition, it has been demonstrated that Id1 is related with TGF-β and Smad signaling in various biological conditions. In this study, we investigated the effect of Id1 on TGF-β-induced collagen expression in human dermal fibroblasts. When Id1-b isoform was overexpressed, TGF-β-induced collagen expression was markedly inhibited. Consistent with this result, Id1-b significantly inhibited TGF-β-induced collagen gel contraction. In addition, Id1-b inhibited TGF-β-induced phosphorylation of Smad2 and Smad3. Finally, immunohistochemistry showed that Id1 expression was decreased in fibrotic skin diseases while TGF-β signaling was increased. Together, these results suggest that Id1 is an inhibitory regulator on TGF-β-induced collagen expression in dermal fibroblasts. |
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Keywords: | Id1 TGF-β Fibroblasts Collagen |
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