Sequencing Studies of Icr-170 Mutagenic Specificity in the am (Nadp-Specific Glutamate Dehydrogenase) Gene of NEUROSPORA CRASSA |
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| Authors: | Philip A. Burns Jane H. Kinnaird Brian J. Kilbey John R. S. Fincham |
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| Affiliation: | Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada;Department of Genetics, University of Edinburgh, King''s Buildings, Edinburgh EH9 3JN, Scotland;Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, England |
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| Abstract: | The acridine half-mustard ICR-170-induced reversion of the mutant am15, which has a single base-pair deletion, at a frequency of between 9 and 28 X 10(-6). In each of three classes of revertants, the mutagen had induced the insertion of a -G- -C- base pair at a -G-G- -C-C- site. The mutant am6, which has a single base pair insertion, is known to be revertible, with UV light, by deletion of a -G- -C- base pair at a -G-G-G- -C-C-C- site. This mutant reverted with ICR-170 at a frequency of 0.1 X 10(-6). These results show that ICR-170 is able to induce addition frameshifts in Neurospora crassa within short, monotonous runs of G:C base pairs, but indicate a lack of deletion activity at such sequences. |
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