Demonstration of rRNA Synthesis in Pre-Gastrular Embryos of Xenopus Laevis |
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Authors: | K. SHIOKAWA Y. MISUMI K. YAMANA |
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Affiliation: | Department of Biology, Faculty of Science, Kyushu University 33, Fukuoka, 812 Japan |
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Abstract: | Isolated cells from Xenopus laevis embryos at various developmental stages were incubated with (methyl-3H) methionine to label newly synthesized RNAs. Methylation of RNAs was studied by analyzing nuclease-digests of high-molecular-weight RNAs by DEAE-Sephadex column chromatography. Labeled rRNA, mRNA and 4s RNA were distinguished by their characteristic patterns of 2'-0-methylation and base-methylation. It was concluded that rRNA synthesis starts during the mid- to late-blastula stage. This is about 4 hr, or at least 3 cell cycles earlier than the initiation of gastrulation, which was previously considered to be the stage when rRNA synthesis begins. |
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