Development of glucose sensor using two-photon adsorbed photopolymerization |
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Authors: | Jong Min Kim Jung-Jin Park Haeng-Ja Lee Woo-Sik Kim Hiroshi Muramatsu Sang-Mok Chang |
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Affiliation: | (1) Department of Chemical Engineering, Dong-A University, 840 Hadan-dong, Saha-Gu, Pusan, 604-714, Korea;(2) Department of Chemical Engineering, ILRI, Kyunghee University, Kyungki-do, 446-701, Korea;(3) Department of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1, Katakura, Hachioji, Tokyo 192-0982, Japan; |
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Abstract: | A novel glucose sensor was constructed, and its analytical potential examined. A chip-type three-electrode system for use in a flow-type electrochemical glucose sensor was fabricated using a UV lithography technique on a glass slide. An Ag/AgCl reference electrode was made by electroplating silver onto a Pt electrode and dipping in a saturated KCl solution for 30 min. In addition, a glucose-sensing electrode was fabricated using a two-photon adsorbed photopolymerization technique with a photo-reactive resin containing a glucose oxidase enzyme, ferrocene mediator, non-ionic surfactant, and carbon nanotubes. The cyclic voltammetry of the potassium ferrocyanide in the Pt sensor system showed a stable electrode condition. The response of the modified Pt sensor confirms the feasibility of using a two-photon adsorbed photopolymerization technique for the easy fabrication of functional biosensors. |
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