In situ removal and purification of biosurfactants by automated surface enrichment |
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Authors: | Julia Glazyrina Stefan Junne Peter Thiesen Klaus Lunkenheimer Peter Goetz |
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Institution: | 1.Departamento de Engenharia Química,Universidade Federal de S?o Carlos,S?o Carlos,Brazil;2.Vallée S.A.,S?o Paulo,Brazil |
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Abstract: | Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, was cultivated in a 5-L stirred and aerated bioreactor under different dissolved
oxygen tensions (0%, 5%, and 30% of saturation) for evaluation of the influence of oxygen on cell growth as well as on the
production of the main antigenic component of the vaccine against erysipelas, a 64–69 kDa protein (SpaA). The microorganism
presented different growth profiles for different aeration conditions. However, at the end of the batch cultivations, similar
cell concentrations were obtained under the studied conditions. In order to maximize biomass titers and antigen production,
the microorganism was cultivated in fed-batch operation mode under aerobic conditions. Under this condition, there was a fivefold
increase in biomass production in comparison to the results attained in batch cultivations. To follow up antigen expression,
samples collected during batch cultivations were concentrated and treated with choline for antigen extraction. Antigen expression
was then assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by murine immunization tests. It was observed
a direct influence of oxygen availability upon antigen expression, which is favored in the presence of oxygen. Analysis of
the samples collected throughout the fed-batch process also revealed that antigen production is growth associated. |
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Keywords: | E rhusiopathiae Bioreactor cultivation Swine erysipelas vaccine Aeration conditions |
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