Production of first and second generations aposporous gametophytes fromPyrrosia piloselloides (L.) Price frond strips cultured in vitro |
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Authors: | S H Kwa Y C Wee C S Loh |
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Institution: | (1) Department of Botany, National University of Singapore, Lower Kent Ridge Road, 0511 Singapore |
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Abstract: | Summary Second generation aposporous gametophytes were obtained from sporophytes derived from first generation aposporous gametophytes, which in turn came from the mature fronds grown from spores in the laboratory. Murashige and Skoog modified medium in 1% agar supplemented with sugar alcohols (sorbitol, mannitol), auxins (NAA, 2,4-D) and cytokinin (BA) promoted a higher percentage of aposporous development from mature fronds ofPyrrosia piloselloides derived from aseptically cultured spores as compared with those obtained from plants in the field. A method using 4 6-diamidino-2-phenyl indole and fluorescence microscopy correlated the deoxyribonucleic acid contents of the aposporous gametophytes and sporophytes derived from them with their ploidy level.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- DAPI
4 6-diamidino-2-phenyl indole
- DNA
deoxyribonucleic acid
- MS
Murashige and Skoog medium
- BA
benzyladenine
- NAA
1-naphthaleneacetic acid |
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Keywords: | Pyrrosia piloselloides apospory gametophyte |
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