Sequence and expression of endogenous S-locus glycoprotein genes in self-compatible Brassica napus |
| |
Authors: | Laurian S Robert Sharon Allard Tanya M Franklin and Martin Trick |
| |
Institution: | (1) Plant Research Centre, Central Experimental Farm, K1A 0C6 Ottawa, Ontario, Canada;(2) John Innes Centre, Cambridge Laboratory, Institute of Plant Science Research, NR4 7UJ Norwich, UK;(3) Present address: Centre for Food and Animal Research, Central Experimental Farm, K1A 0C6 Ottawa, Ontario, Canada |
| |
Abstract: | Brassica napus is an amphidiploid plant which is self-compatible even though it is derived from hybridisation of the self-incompatible species B. oleracea and B. campestris. Experiments were undertaken to establish if S-locus glycoprotein (SLG) genes exist in B. napus and whether these are expressed as in self-incompatible Brassica species. Two different stigma-specific cDNA sequences homologous to SLG genes were obtained from the B. napus cultivar Westar. One of these sequences, SLG
WS1, displayed highest homology to class I SLG alleles, whereas the other, SLG
WS2, showed greatest homology to class II SLG genes. Both were expressed at high levels in Westar stigmas following a developmental pattern typical of SLG genes in the self-incompatible diploids. We infer that they represent the endogenous SLG genes at the two homoeologous S-loci. The occurrence of normally expressed SLG genes and its relevance to the self-compatible phenotype of B. napus is discussed. |
| |
Keywords: | Brassica Self-incompatibility Self-compatibility S-locus glycoprotein |
本文献已被 SpringerLink 等数据库收录! |
|