Assignment of 82 Known Genes and Gene Clusters on the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803 |
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Authors: | Kotani Hirokazu; Tanaka Ayako; Kaneko Takakazu; Sato Shusei; Sugiura Masahiro; Tabata Satoshi |
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Institution: | 1Kazusa DNA Research Institute Yanauchino, Kisarazu, Chiba 292, Japan
2Center for Gene Research, Nagoya University Furoh-cho, Nagoya 464-01, Japan |
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Abstract: | We have previously constructed the physical map of a cyanobacterium,Synechoystis sp. strain PCC6803 on the basis of restrictionand linking clone analysis. Since a total of 82 genes and geneclusters have been isolated from this strain, most of whichare involved in oxygenic photosynthesis, portions of their sequenceswere amplified by the PCR method and assigned on the physicalmap of the genome by hybridization with restriction fragments,ordered clones, which were obtained from cosmid and libraries,and long PCR-products. An exception was the gene psbG2 whichwas mapped on an extra-chromosomal unit of 45 kb. Since geneticmaps of some of genes assigned above, especially those for photosynthesis,have been reported for two other cyanobacterial strains, Anabaenasp. PCC7120 and Synechococcus sp. PCC7002, gene organizationswere compared among the three strains. However, no significantcorrelation was observed, suggesting that rearrangement of genesoccurred in the respective strains during or after establishmentof the species. |
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Keywords: | Synechocystis PCC6803 Genome map Ordered clones |
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