Purification and characterization of trypsin-like enzymes from North Pacific krill (<Emphasis Type="Italic">Euphausia pacifica</Emphasis>) |
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Authors: | Zhiqiang Wu Guoliang Jiang Peng Xiang Dong Yang Ning Wang |
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Institution: | (1) College of Marine Life Science, Ocean University of China, Qingdao, 266003, China |
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Abstract: | Two trypsin-like enzymes (TLEs) were purified from North Pacific krill (Euphausia pacifica) by ammonium sulfate precipitation, ion-exchange and gel-filtration chromatography. The purified enzymes were identified
as trypsins by LC-ESI-MS/MS analysis. The relative molecular mass of TLE I and TLE II were 33 and 32.3 kDa, respectively,
with isoelectric points of 4.5 and 4.3, respectively. The TLEs showed excellent thermal stable in the crude extract and the
purified TLEs were active over a wide pH (6.0–11.0) and temperature (10–70°C) range. Compared with trypsins from other organisms,
the purified TLEs had physiological efficiencies of 1.6–6.7-fold. The difference in Arg, Ile and Asp content might explain
why E. pacifica TLEs have good thermal stability and physiological efficiency. |
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Keywords: | Amino acid composition Euphausia pacifica Physiological efficiency Stability Trypsin-like enzyme |
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