Liquid holding recovery and photoreactivation of UV-induced damage inStreptococcus lactis |
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Authors: | Martine Lautier Bouachanh Thammavongs Yanick Auffray Philippe Boutibonnes |
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Institution: | (1) Laboratoire de Génétique Microbienne, Equipe de Toxicologie Génétique, Institut de Recherche de Biologie Appliquée (I.R.B.A.), Université de Caen, 14032 Caen, France |
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Abstract: | Summary Repair of ultraviolet-light-induced DNA damage inStreptococcus lactis has been examined. The wild-type strain and its derivative Lac– possess a dark repair system (maximal increase in survival of 4-fold). Enzymatic photoreactivation exists in the two strains but a weaker photoreactivability was found in the Lac– derivative (4 and 2-fold, respectively). Concomitant reduction of UV-induced mutagenesis (Rifr marker) was also studied during these two repair phenomena. The absence of dark repair after saturation of photoreactivation suggests that photoreactivation is much more efficient with pyrimidine dimers as substrate. |
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Keywords: | Streptococcus lactis UV irradiation Liquid holding recovery Photoreactivation |
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