| 人MCP cDNA的克隆、序列分析及同种型的比较 |
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| 引用本文： | 黄健,李文鑫,苟德明,蒋达和.人MCP cDNA的克隆、序列分析及同种型的比较[J].生物化学与生物物理进展,1999,26(4):355-358. |
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| 作者姓名： | 黄健 李文鑫 苟德明 蒋达和 |
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| 作者单位： | 武汉大学生命科学学院,武汉430072 |
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| 基金项目： | 湖北省95重点科技攻关计划项目资助课题(96192004) |
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| 摘 要： | 以人胚胎mRNA为模板，采用RT-PCR法得到了人补体调节蛋白膜辅蛋白(MCP)的一种cDNA全基因，序列分析结果表明，所获得的MCP cDNA为文献报道中10种同种型中的一种，属MCP-C2型，该cDNA含一编码369个氨基酸的阅读框架，其中的STP区含14个氨基酸，由STPC编码，胞浆尾区含23个氨基酸，为CYT2,未发现有东西方人种之间的核苷酸的差异.
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| 关 键 词： | 膜辅蛋白，MCP cDNA，序列分析，可变剪接的RNA，同种型 |
| 收稿时间： | 3/2/1998 12:00:00 AM |
| 修稿时间： | : 1998-03- |
Cloning, Sequencing of Human Membrane Cofactor Protein (MCP) and Comparison with Other Isoforms |
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| HUANG Jian,LI Wen-Xin,GOU De-Ming and JIANG Da-He.Cloning, Sequencing of Human Membrane Cofactor Protein (MCP) and Comparison with Other Isoforms[J].Progress In Biochemistry and Biophysics,1999,26(4):355-358. |
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| Authors: | HUANG Jian LI Wen-Xin GOU De-Ming and JIANG Da-He |
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| Institution: | College of Life Sciences,Wuhan University, Wuhan 430072, China;College of Life Sciences,Wuhan University, Wuhan 430072, China;College of Life Sciences,Wuhan University, Wuhan 430072, China;College of Life Sciences,Wuhan University, Wuhan 430072, China |
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| Abstract: | MCP is an important membrane protein for protecting host cells from damage by complement because of its wide tissue distribution and cofactor activity. A human MCP cDNA containing full encoding region was obtained by RT-PCR from Chinese human embryo. The target gene was cloned and sequenced. The data indicated that there are forty-five bases deletion compared with the counterparts previouly reported,which leads to the deletion of fifteen amino acid residues. The PCR product including 369aa open reading frame was one of the ten isoforms which had been reported before and it was named MCP-C2. |
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| Keywords: | MCP cDNA RT-PCR DNA sequencing alternative splicing isoforms |
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