Ammonia production from yeast extract and its effect on growth of the hyperthermophilic archaeonSulfolobus solfataricus |
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Authors: | Chan Beum Park Sun Bok Lee |
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Affiliation: | (1) Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, 790-784 Pohang, Korea |
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Abstract: | Utilization of yeast extract and formation of byproduct metabolite were investigated for hyperthermophilic archaeonSulfolobus solfataricus (DSM 1617). In both batch and fed-batch cultivations ofS. solfataricus, maximal cell density, NH4 + ion production and pH change were highly dependent on the ratio of yeast extract to glucose in the medium. Variation of NH4 + ion level was identified as a major cause of pH change during cultivation, and acidification of culture broth was attributed to consumption of NH4 + ions rather than formation of acid byproducts. It was also observed that increase of NH4 + ion concentrations in the medium resulted in greater degree of growth inhibition. |
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Keywords: | hyperthermophile archaea Sulfolobus solfataricus ammonia yeast extract fed-batch |
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