| 人血小板生成素(hTPO)全长 cDNA 克隆及在 CHO 细胞中的表达 |
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| 引用本文： | 邢桂春, 郭树华, 胡志远, 张咏, 董波, 贺福初,. 人血小板生成素(hTPO)全长 cDNA 克隆及在 CHO 细胞中的表达[J]. 生物工程学报, 1997, 13(4) |
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| 作者姓名： | 邢桂春 郭树华 胡志远 张咏 董波 贺福初 |
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| 作者单位： | 北京放射医学研究所; |
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| 基金项目： | 863计划,自然科学基金 |
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| 摘 要： | 以人胎肝ｍＲＮＡ为模板，采用ＲＴ-ＰＣＲ扩增了人ＴＰＯ编码区全长ｃＤＮＡ，全序列测定结果表明与国外报道序列一致；进而构建了ｐｃＤＮＡ３-ＴＰＯ永久表达载体，转染ＣＨＯ细胞后经Ｇ４１８加压筛选、ＴＰＯ表达稳定性等项指标测试，获得了稳定分泌ＴＰＯ的工程细胞株。
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| 关 键 词： | 人血小板生成素 PCR 序列分析 CFU-Meg CHO细胞表达 |
Cloning and Expression of a Full Length cDNA of Human Thrombopoietin (hTPO)in CHO Cells |
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| Xing Guichun Guo Shuhua Hu Zhiyuan Zhang Yong Dong Bo He Fuchu. Cloning and Expression of a Full Length cDNA of Human Thrombopoietin (hTPO)in CHO Cells[J]. Chinese journal of biotechnology, 1997, 13(4) |
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| Authors: | Xing Guichun Guo Shuhua Hu Zhiyuan Zhang Yong Dong Bo He Fuchu |
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| Abstract: | A full length cDNA of the coding region of hTPO,whose sequence is identical to those previously reported,has been amplified by means of RT PCR with human fetal liver mRNA as the templates and then sequenced.Further,this insert was subcloned into a mammalian expression vector pcDNA3 (pcDNA3 TPO),transformed into CHO cells, and screened by G418 and then tested by expression level of hTPO.A group of stably secreting engineered strains of hTPO has been obtained. |
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| Keywords: | Human thrombopoietin CFU Meg CHO cell PCR sequencing analysis expression |
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