Discrimination of higher plant calluses based on embryogenic capacity and taxonomic classification by pyrolysis mass spectrometry |
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Authors: | Suk Weon Kim Sung Hee Ban Ook Joon Yoo Jang Ryol Liu |
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Institution: | (1) Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoeun-dong, Yuseong-gu, Daejeon, 305-333, South Korea;(2) Department of Biological Science, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yuseong-gu, Daejeon, 305-701, South Korea;(3) Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoeun-dong, Yuseong-gu, Daejeon, 305-333, South Korea |
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Abstract: | Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex
material in a vacuum, and has been widely applied to the discrimination of closely related microbial strains. Minimally prepared
samples of embryogenic and non-embryogenic calluses derived from various higher plants (sweet potato, morning glory, Korean
ginseng, Siberian ginseng, and balloon flower) were subjected to PyMS for spectral fingerprinting. A dendrogram based on the
unweighted pair group method, with arithmetic mean of pyrolysis mass spectra, divided the calluses into Siberian ginseng embryogenic
callus and the others, which were subsequently divided into embryogenic and non-embryogenic callus groups, regardless of plant
species from which the calluses were derived. In the non-embryogenic callus group, the dendrogram was in agreement with the
known taxonomy of the plants. These results indicate that PyMS analysis could be applied for discriminating plant calluses
based on embryogenic capacity and taxonomic classification. |
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Keywords: | Canonical variate analysis Multivariate analysis Principal component analysis |
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