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Immunogenicity of outer membrane derivatives ofHaemophilus influenzae type b
Authors:Joseph S. Lam  Dan M. Granoff  Janet R. Gilsdorf  J. William Costerton
Affiliation:(1) Department of Biology, University of Calgary, T2N 1N4, Alberta, Canada;(2) Present address: Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, 63178 St. Louis, Missouri, USA;(3) Division of Infectious Diseases, St. Louis Children's Hospital, 63178 St. Louis, Missouri, USA;(4) Department of Pediatrics, University of Minnesota, Mayo Building, 420 Delaware Street, S.E., Box 296, 55455 Minneapolis, Minnesota, USA
Abstract:We prepared outer membrane derivatives ofHaemophilus influenzae type b to determine whether the residual capsular and noncapsular surface components are immunogenic and protective. These fragments consist primarily of six major proteins and lipopolysaccharide. By transmission electron microscopy, they appeared as small, membrane-like fragments or larger, cellshaped double-track ghosts. Rabbits immunized with ghosts responded with increases in serum anticapsular antibody and bactericidal activity. Antisera absorbed with capsular antigen to remove anticapsular antibody remained bactericidal and passively protected infant rats. These data suggest that antibodies to noncapsular surface antigens are protective, and that outer membrane derivatives retain some of the constituents responsible for stimulating immunity.
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