| 环氧丙醇酯立体专-性水解酶产生菌的筛选 |
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| 引用本文： | 贾师英,李青山,许建和,俞俊棠.环氧丙醇酯立体专-性水解酶产生菌的筛选[J].微生物学通报,1999(2). |
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| 作者姓名： | 贾师英 李青山 许建和 俞俊棠 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室!上海２００２３７ |
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| 基金项目： | 山东大学微生物技术国家重点实验室开放课题 |
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| 摘 要： | 从土壤中筛选出一株能拆分（Ｒ，Ｓ）－环氧丙醇丁酸酯的根霉（Ｒｈｉｚｏｐｕｓｓｐ．Ｂｃ０－０９），该菌株所产胞外脂肪酶在水解环氧丙醇丁酸酯的反应中具有良好的立体专一性。在ｐＨ恒定７．０的条件下，以其发酵液水解底物，当转化率为５８％时，残留的（Ｒ）－环氧丙醇丁酸酯的光学纯度（对映体过量值）达９６．１％。
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| 关 键 词： | （R，S）－环氧丙醇丁酸酯 光学拆分 脂肪酶 根霉 筛选 |
SCREENING OF LIPASE PRODUCING STRAIN FOR STEREOSPECIFIC HYDROLYSIS OF (R,S) -GLYCIDYL BUTYRATE |
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| Jia Shiying, Li Qingshan , Xu Jianhe , Yu Juntang.SCREENING OF LIPASE PRODUCING STRAIN FOR STEREOSPECIFIC HYDROLYSIS OF (R,S) -GLYCIDYL BUTYRATE[J].Microbiology,1999(2). |
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| Authors: | Jia Shiying Li Qingshan Xu Jianhe Yu Juntang |
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| Abstract: | A strain of microorganism capable of optically resolving racemic glycidyl butyrate has been isolatedfrom soil and identified to be a Rhizopus sp. (Bc0-09). The preliminary investigation on the optical resolution ofglycidyl butyrate showed that the lipase from Rhizopus sp. was able to stereoselectively hydrolyze (S)-enantiomerof the racemic ester. When the racemic substrate was enzymatically hydrolyzed to 58% conversion at constantpH(7.0), the optical purity of recovered (R)-ester was 96.1% ee (enantiomeric excess). |
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| Keywords: | Rhizopus sp lipese screening (R S)-glycidyl butyrate Optical resolution |
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