Localization of tRNA genes of Drosophila melanogaster by in situ hybridization |
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Authors: | S Hayashi I C Gillam T A Grigliatti G M Tener |
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Institution: | (1) Department of Biochemistry, University of British Columbia, V6T 1W5 Vancouver, B.C., Canada;(2) Department of Zoology, University of British Columbia, V6T 1W5 Vancouver, B.C., Canada |
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Abstract: | Six purified tRNAs labeled with 125I by chemical or enzymatic methods were hybridized to polytene chromosomes of Drosophila melanogaster. The main chromosomal regions of hybridization were: tRNA
GGA
Gly
, 58A, 84C, and 90E; tRNA
2
Leu
, 44E, 66B5-8, and 79F; tRNA
2b
Ser
, 86A, 88A9-12, and 94A6-8; tRNA
3
Thr
, 47F and 87B; tRNA
4
Thr
, 93A1-2; and tRNA
1
Tyr
, 19F, 22F-23A, 41, 50C1-4 and 85A. At 50C the hybridization of tRNA
1
Tyr
was polymorphic in the giant strains. When the hybridization of three valine isoacceptors studied previously was re-investigated, it was found that only one hybridization site, 90BC, was shared between tRNA
3b
Val
and tRNA
4
Val
. tRNA
3a
Val
did not have any sites in common with the other two. |
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Keywords: | |
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