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Isolation, characterization and molecular cloning of new temporins from the skin of the North African ranid Pelophylax saharica
Authors:Abbassi Feten  Oury Bruno  Blasco Thierry  Sereno Denis  Bolbach Gérard  Nicolas Pierre  Hani Khaled  Amiche Mohamed  Ladram Ali
Institution:FRE 2852, Peptidome de la Peau des Amphibiens, Université Pierre et Marie Curie/CNRS, Tour 43, 2 place Jussieu, 75251 Paris Cedex 05, France; Laboratoire de Biochimie, Faculté de Médecine de Sousse, 4002 Sousse, Tunisia.
Abstract:Temporins are small antimicrobial peptides isolated from North American and Eurasian ranid frogs that are particularly active against Gram-positive bacteria. To date, no temporins have been characterized from North African frog species. We isolated three novel members of the temporin family, named temporin-1Sa (FLSGIVGMLGKLF(amide)), -1Sb (FLPIVTNLLSGLL(amide)), and -1Sc (FLSHIAGFLSNLF(amide)), from the skin of the Sahara frog Pelophylax (Rana) saharica originating from Tunisia. These temporins were identified by a combined mass spectrometry/molecular cloning approach. Temporin-1Sa was found to be highly active against Gram-positive and Gram-negative bacteria, yeasts and fungi (MIC=2-30muM). To our knowledge, this is the first 13-residue member of the temporin family with a net charge of +2 that shows such broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC=10muM) and Pseudomonas aeruginosa (MIC=31muM). Moreover, temporin-1Sa displays significant antiparasitic activity (IC(50) approximately 20muM) against the promastigote and amastigote stages of Leishmania infantum.
Keywords:Amphibian  Pelophylax (Rana) saharica  Temporins
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