A novel mitochondrial DEAD box protein (Mrh4) required for maintenance of mtDNA in Saccharomyces cerevisiae |
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Authors: | Schmidt Udo Lehmann Karola Stahl Ulf |
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Institution: | Department of Microbiology and Genetics, Faculty III, University of Technology Berlin, Gustav-Meyer-Allee 25, Germany. u.schmidt@lb.tu-berlin.de |
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Abstract: | In a screen of nuclear genes that assist splicing of mitochondrial localized group II introns in yeast we isolated low-copy number suppressors of splicing and respiratory-deficient point mutants of intron aI5gamma, the last intron of the gene encoding cytochrome c oxidase subunit I. One of the genes found contains the open reading frame (ORF) YGL064c that has previously been proposed to encode a putative RNA helicase of the DEAD box family. Deletion of the ORF gives rise to 100% cytoplasmic petites, indicating that the protein plays an essential role in the mitochondrial RNA metabolism. Overexpression of YGL064c-GFP fusions clearly revealed a mitochondrial localization of the protein. The gene encodes the fourth putative RNA helicase of Saccharomyces cerevisiae implicated in a mitochondrial function and was therefore termed MRH4 (for mitochondrial RNA helicase). |
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Keywords: | YGL064c MRH4 Mitochondrial DEAD box protein RNA helicase Mitochondrial DNA stability Group II intron Nuclear suppressor |
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