Simple and efficient protocol for isolation of high molecular weight DNA from Streptomyces aureofaciens

A simple and efficient protocol involving the use of cetyltrimethylammonium bromide (CTAB) for rapid isolation of high molecular wt ( 50 kb) DNA from Streptomyces aureofaciens is described. The DNA yields range from 1.5–2.5 mg per 1.0 g (wet wt) of mycelia, with the purity measured at A260/280 of 1.83–1.97 and also at A260/230 of 2.2–2.71. The DNA preparation is suitable as substrate for restriction digestion, Southern hybridization and library construction. © Rapid Science Ltd. 1998