An efficient plant regeneration system for Mucuna pruriens L. (DC.) using cotyledonary node explants |
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Authors: | M Faisal I Siddique M Anis |
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Institution: | (1) Plant Tissue Culture Laboratory, Department of Botany, Aligarh Muslim University, 202 002 Aligarh, India |
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Abstract: | Summary The purpose of this study was to develop an efficient micropropagation system for Mucuna pruriens, an important medicinal plant in India. A range of cytokinins was investigated for multiple shoot regeneration with cotyledonary
node explants from 7-d-old aseptic seedlings. Of all the cytokinins, 6-benzyladenine (BA), kinetin (KIN) and 2-isopentenyl
adenine (2-iP) tested in Murashige and Skoog medium (MS), BA was the most effective and 5.0 μM was found to be optimum for inducing maximum shoots. Medium types, medium strength and pH were also investigated for induction
and proliferation of shoots. The highest efficiency of shoot proliferation was observed in 5.0 μM BA and 0.5 μM α-naphthalene acetic acid (NAA) in half-strength MS medium at pH 5.8. The best condition for rooting was half-strength MS
medium solidified with agar and with 2.0 μM indole-3-butyric acid (IBA). After rooting, the plantlets were transferred to plastic pots filled with sterile soilrite where
90% grew and all exhibited normal development. |
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Keywords: | acclimatization cotyledonary node Mucuna pruriens micropropagation multiple shoots |
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