AMSH regulates calcium-sensing receptor signaling through direct interactions |
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Authors: | Herrera-Vigenor Francisco Hernández-García Ricardo Valadez-Sánchez Margarita Vázquez-Prado José Reyes-Cruz Guadalupe |
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Institution: | Department of Cell Biology, CINVESTAV-IPN Apartado postal 14-740, México DF 07000, Mexico. |
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Abstract: | Calcium-sensing receptor (CaR) activates intracellular pathways controlling calcium homeostasis. CaR carboxyl-terminal mutants associated with metabolic diseases suggest that unidentified proteins interact with the carboxyl-terminal region of this receptor. To address this possibility, we screened for CaR-interacting proteins using the carboxyl terminus of CaR (CaRDelta895-1075 deletion mutant). We identified AMSH, an ubiquitin isopeptidase, as a CaR-interacting partner. AMSH caused a decrease on the signaling properties of wild-type and mutant CaR. Our results indicate that AMSH, which has been recently characterized as a regulator of the endosomal sorting of epidermal growth factor receptor, represents a novel modulator of CaR signaling. |
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Keywords: | Calcium-sensing receptor G-protein-coupled receptors AMSH Ubiquitin isopeptidase |
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