Identification of NGF receptor in chromatin of melanoma cells using monoclonal antibody to cell surface NGF receptor |
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| Authors: | E M Rakowicz-Szulczynska H Koprowski |
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| Affiliation: | 1. Department of Civil Engineering, Hefei University of Technology, Hefei 230009, PR China;2. The Key Laboratory of Urban Security and Disaster Engineering of Ministry of Education, Beijing University of Technology, Beijing 100124, PR China;1. Department of Earth, Environmental and Planetary Sciences, Brown University, Providence, RI, 02912, USA;2. The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA, 02543, USA;3. Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, AB Den Burg, the Netherlands;4. Department of Freshwater and Marine Ecology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, the Netherlands;5. Centro de Investigação em Biodiversidade e Recursos Genéticos, CIBIO, InBIO Laboratório Associado, Pólo dos Açores, Portugal;6. Faculdade de Ciências e Tecnologia, Universidade dos Açores, Ponta Delgada, Açores, Portugal;7. CREAF & UAB, Cerdanyola del Vallès, Barcelona, Spain;8. Department of Geography, Environmental Management and Energy Studies, University of Johannesburg, Corner Ditton and University Avenue, Auckland Park, Johannesburg, South Africa;9. Universidade da Coruña, GRICA Group, Centro de Investigacións Científicas Avanzadas (CICA), Rúa as Carballeiras, 15071, A Coruña, Spain;10. Geosciences Barcelona (GEO3BCN-CSIC), Spanish National Research Council, Barcelona, Spain;11. UB-Geomodels Research Institute. Department of Earth and Ocean Dynamics, Universitat de Barcelona, Barcelona, Spain;12. Instituto Dom Luiz (IDL), Faculty of Sciences, University of Lisboa, Lisbon, Portugal;13. Marine Research Institute (IIM), Spanish National Research Council (CSIC), Vigo, Spain;1. Universidad de Sevilla, Spain;2. Universidad Autónoma de Chile, Chile;1. Department of Computer Engineering, Bergen University College, Norway;2. Department of Mathematics and Informatics, Philipps-University of Marburg, Germany;3. Department of Informatics, University of Bergen, Norway;1. Cytogenetics, Genetics and Plant Breeding Section, Department of Botany, Kalyani University, Kalyani, 741235 West Bengal, India;2. Pteridology and Palaeo-botany Section, Department of Botany, Kalyani University, Kalyani, 741235 West Bengal, India |
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| Abstract: | A 230 KDa species of Nerve Growth Factor (NGF) receptor was immunoprecipitated from EcoRI-digested chromatin of melanoma cells using a monoclonal antibody to the 75 KDa cell surface NGF receptor. The chromatin NGF receptor was shown to exist tightly bound to DNase II-sensitive sequences which, upon growth factor binding, became resistant to DNase II digestion. |
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