Presence of corazonin in three insect species, and isolation and identification of [His7]corazonin from Schistocerca americana. |
| |
Authors: | Jan A Veenstra |
| |
Institution: | Department of Entomology and Center for Insect Science, The University of Arizona, Tucson, AZ, USA |
| |
Abstract: | An ELISA for corazonin, a cardioactive neuropeptide from the American cockroach, Periplaneta americana, was developed. It was used to isolate corazonin from the cockroach Nauphoeta cinerea, the locust Schistocerca americana, and the hawkmoth Manduca sexta. The peptides from Nauphoeta and Manduca had the same retention times as Periplaneta corazonin, and their amino acid compositions also suggested that these peptides are identical with corazonin. The corazonin-immunoreactive peptide from Schistocerca eluted slightly earlier on HPLC than corazonin, and its structure was determined to be His7]corazonin, or pGlu-Thr-Phe-Gln-Tyr-Ser-His-Gly-Trp-Thr-Asn-amide. These results indicate that corazonin is generally present in insects and that its structure has been well conserved. |
| |
Keywords: | Schistocerca americana Manduca sexta Nauphoeta cinerea Corazonin [His7]Corazonin Cardioactive peptide ELISA |
本文献已被 ScienceDirect 等数据库收录! |
|