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A non-enzymatic method of preparing isolated hepatocytes: assessment of their morpho-functional activity |
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| Authors: | L P Kravchenko A N Andrienko A Iu Petrenko A N Sukach |
| Abstract: | A method for isolating hepatocytes using the two-step liver perfusion by chelate-containing solutions is described. The hepatocytes obtained are highly inactive for the trypan blue staining test, (not less than 90%), ATP content, the rate of endogenic respiration and its stimulation by succinate, a degree of xenobiotic oxidation. |
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