Identification of the regions of Fv1 necessary for murine leukemia virus restriction |
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Authors: | Bishop K N Bock M Towers G Stoye J P |
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Affiliation: | Division of Virology, National Institute for Medical Research, London NW7 1AA, United Kingdom. |
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Abstract: | The Fv1 gene restricts murine leukemia virus replication via an interaction with the viral capsid protein. To study this interaction, a number of mutations, including a series of N-terminal and C-terminal deletions, internal deletions, and a number of single-amino-acid substitutions, were introduced into the n and b alleles of the Fv1 gene and the effects of these changes on virus restriction were measured. A significant fraction of the Fv1 protein was not required for restriction; however, retention of an intact major homology region as well as of domains toward the N and C termini was essential. Binding specificity appeared to be a combinatorial property of a number of residues within the C-terminal portion of Fv1. |
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