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| 猪血超氧化物歧化酶分离纯化工艺改进及其抗氧化活性研究 | |
| 引用本文： | 王保全,庞晓斌,李昭华,平娟,王玲玲,董先智. 猪血超氧化物歧化酶分离纯化工艺改进及其抗氧化活性研究[J]. 生物技术通报, 2010, 0(2) |
| 作者姓名： | 王保全 庞晓斌 李昭华 平娟 王玲玲 董先智 |
| 作者单位： | 1. 河南大学药学院,开封,475001;中国科学院生物物理研究所,北京,100101 2. 河南大学药学院,开封,475001 3. 中国科学院生物物理研究所,北京,100101 |
| 摘 要： | 以新鲜猪血为原料,首次利用改进的新工艺提取分离超氧化物歧化酶,经过溶血,热变,丙酮沉淀,超滤浓缩,SephadexG-75凝胶过滤层析和DEAE-sepharose-fast flow离子交换层析纯化,冷冻干燥等步骤,得到高纯度酶,并对酶的相关性能进行研究。试验结果显示产品粗酶活性在3 000 U/mg左右,分别经SephadexG-75和DEAE-sepharose-fast flow层析纯化后,酶活分别达到5 585 U/mg和6 148 U/mg产品得率分别为13.4%和10.32%,SDS-PAGE凝胶电泳显示为单一条带达到电泳纯,其分子量在31 kD附近,其临苯三酚抗氧化活性明显。 |
| 关 键 词： | 超氧化物岐化酶 新工艺 抗氧化 |
The Process Improvement on the Separation and Purification of Superoxide Dismutase from Pig Blood and Its Oxidation Resistance |
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| Wang Baoquan,Pang Xiaobin,Li Zhaohua,Ping Juan,Wang Lingling,Dong Xianzhi. The Process Improvement on the Separation and Purification of Superoxide Dismutase from Pig Blood and Its Oxidation Resistance[J]. Biotechnology Bulletin, 2010, 0(2) | |
| Authors: | Wang Baoquan Pang Xiaobin Li Zhaohua Ping Juan Wang Lingling Dong Xianzhi |
| Affiliation: | Wang Baoquan1.2 Pang Xiaobin 1 Li Zhaohua 2 Ping Juan1 Wang Lingling1 Dong Xianzhi 2(1Pharmaceutical College of Henan University,Kaifeng 475001,2Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101) |
| Abstract: | The Superoxide dismutase(SOD)was extracted from fresh pig blood by improved process via the key step such as hemolysis,heat denaturation,ultrafiltration concentration,lyophilization,sephadexG-75 gel permeation chromatography and DEAE-sepharose-fast-flow ion-exchange chromatography.High purity enzyme was obtained and the related properties were studied.The results showed that the activity of the rude enzyme is about 3 000 U/mg,its activity reaches 5 585 U/mg,6 148 U/mg and the yield rate is 13.4%,10.32% afte... |
| Keywords: | Superoxide dismutage New process Oxidation resistanceo |
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