| HPLC-UV法定量测定发酵液中的S-腺苷-L-甲硫氨酸 |
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| 引用本文： | 蔡恒,郑伟刚,万红贵,郭一丹,王涛.HPLC-UV法定量测定发酵液中的S-腺苷-L-甲硫氨酸[J].中国生物工程杂志,2007,27(11):73-76. |
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| 作者姓名： | 蔡恒 郑伟刚 万红贵 郭一丹 王涛 |
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| 作者单位： | 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 |
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| 摘 要： | 本文采用高效液相色谱-紫外检测器（HPLC-UV）定量测定发酵液中S-腺苷-L-甲硫氨酸（SAM）的含量。结果表明，S-腺苷-L-甲硫氨酸浓度在0.1~1.0g/L时，其峰面积（Y）与相应的浓度（X）呈线性关系，线性方程为Y=13.937X－0.1949，相关系数为0.9969。S-腺苷-L-甲硫氨酸的平均回收率为99.89~101.7%，相对标准偏差为0.48~1.36%。该方法精密度、准确度好，稳定性高，能简便、快速、准确地测定发酵液中S-腺苷-L-甲硫氨酸的含量。
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| 关 键 词： | S-腺苷-L-甲硫氨酸 HPLC UV |
| 收稿时间： | 2007-05-29 |
| 修稿时间： | 2007-09-24 |
Detection of S-Adenosyl-L-methionine in Fermentation Liquid by HPLC-UV |
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| CAI Heng,ZHENG Wei-gang,WAN Hong-gui,GUO Yi-dan,WANG Tao.Detection of S-Adenosyl-L-methionine in Fermentation Liquid by HPLC-UV[J].China Biotechnology,2007,27(11):73-76. |
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| Authors: | CAI Heng ZHENG Wei-gang WAN Hong-gui GUO Yi-dan WANG Tao |
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| Institution: | College of Pharmacy and Life Science,Nanjing University of Technology, Nanjing 210009, China |
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| Abstract: | This paper developed a method for the determination of S-Adenosyl-L-methionine in fermentation liquid using the high performance liquid chromatography coupled with UV detector (HPLC-UV). A good linearity was obtained in the range of 0.1~1.0g/L (r = 0.9969) for S-Adenosyl-L-methionine. The average recoveries of S-Adenosyl-L-methionine were 99.89~101.7% and the relative standard deviations were 0.48~1.36%.The method is simple and rapid with reproducibility for the determination of S-Adenosyl-L-methionine in
fermention liquid. |
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| Keywords: | HPLC UV |
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