Sennosides A and B production by hairy roots of Senna alata (L.) Roxb |
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Authors: | Putalun Waraporn Pimmeuangkao Suwat De-Eknamkul Wanchai Tanaka Hiroyuki Shoyama Yukihiro |
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Institution: | Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. waraporn@kku.ac.th |
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Abstract: | Hairy roots of Senna alata transformed with Agrobacterium rhizogenes, strain ATCC 15834 were induced and grown in half-strength Murashige and Skoog (MS) medium. Effects of sucrose contents and hormones on the growth and sennosides A, B production were investigated. Hairy roots cultured on hormone-free half-strength MS medium containing 5% sucrose under dark condition mostly stimulated the growth of hairy roots and increased the content of sennosides A and B yielding (169 +/- 4) and (34 +/- 3) microg g(-1) dry wt, respectively. |
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