39K nuclear magnetic resonance and a mathematical model of K+ transport in human erythrocytes |
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Authors: | Anthony D. Maher Bogdan E. Chapman Philip W. Kuchel |
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Affiliation: | (1) School of Molecular and Microbial Biosciences, University of Sydney, 2006 Sydney, NSW, Australia |
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Abstract: | 39K nuclear magnetic resonance was used to measure the efflux of K+ from suspensions of human erythrocytes [red blood cells (RBCs)], that occurred in response to the calcium ionophore, A23187 and calcium ions; the latter activate the Gárdos channel. Signals from the intra- and extracellular populations of 39K+ were selected on the basis of their longitudinal relaxation times, T 1, by using an inversion- recovery pulse sequence with the mixing time, τ1, chosen to null one or other of the signals. Changes in RBC volume consequent upon efflux of the ions also changed the T 1 values so a new theory was implemented to obviate a potential artefact in the data analysis. The velocity of the K+ efflux mediated by the Gárdos channel was 1.19±0.40 mmol (L RBC)−1 min−1 at 37°C. |
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Keywords: | 39K nuclear magnetic resonance K+ transport Erythrocyte Membrane transport Mathematica |
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