Recombinant Protein Purification using Composite Polyacrylamide-Nanocrystalline Cryogel Monolith Column and Carbohydrate-Binding Module Family 64 as Affinity Tag |
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Authors: | Mohsen Danaeifar Ziba Veisi Malekshahi Fatemeh Kazemi-Lomedasht Mohammad Ali Mazlomi |
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Affiliation: | 1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. ; 2. Department of Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. |
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Abstract: | Background:In the field of recombinant protein production, downstream processing, especially protein purification, is critical and often the most expensive step. Carbohydrate binding module 64 (CBM64) was shown in 2011 to bind efficiently to a broad range of cellulose materials.Methods:In this study, we developed a protein purification method using nanocrystalline cellulose embedded in a polyacrylamide monolith cryogel and CBM64 affinity tag linked by intein to PD1 as a model protein. The CBM64-Intein-PD1 gene cassette was expressed in E. coli. Following cell lysis, CBM64-Intein-PD1 protein bound to the monolith PA-NCC cryogel. After washing and reducing the pH from 8.0 to 6.5, the intein underwent self-cleavage, resulting in the release and elution of pure PD1 protein.Results:The synthesized monolith column had a porous structure with an average pore size of 30 µm and a maximum binding capacity of 497 µg per gram of dried column. The yield of this purification method was 84%, while the yield of the His tag-acquired CBM64-Intein-PD1 method was 89%.DiscussionWe used cellulose as support for affinity chromatography, which can be used as a cost-effective method for protein purification.Key Words: Affinity tag, CBM64, PD1, Protein purification |
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