Magnetic force microscopy analysis of apoptosis of HL-60 cells induced by complex of antisense oligonucleotides and magnetic nanoparticles |
| |
Authors: | Shen He-bai Long De-hong Zhu Long-zhang Li Xing-Yu Dong Ya-ming Jia Neng-qin Zhou Hai-qing Xin Xi Sun Yang |
| |
Affiliation: | Life and Environment Science College, Shanghai Normal University, Shanghai 200234, PR China. Shenhb@shnu.edu.cn |
| |
Abstract: | Magnetic force microscopy (MFM) has been employed to observe antisense oligonucleotides (ASOs)-coupled silica-coated magnetic iron oxide nanoparticles (SMNPs) internalized into human leukemia (HL-60) cells. The experiment demonstrated that the ASOs-coupled SMNPs delivery into the cells really occurred. The nanoparticles were internalized into the cells and the apoptotic topography can be directly visualized simultaneously with MFM technology. These present observations offer direct morphology evidence on studying the apoptosis of tumor cells and provide useful information for better design of new diagnostic and therapeutic tools in tumor treatment. |
| |
Keywords: | Human leukemia cells Magnetic force microscopy Morphologic image Magnetic image Antisense oligonucleotides Apoptosis |
本文献已被 ScienceDirect PubMed 等数据库收录! |