Effects of saturated fatty acids on n-6 fatty acid metabolism in cultured human monocyte-like cells (U937) |
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Authors: | A. Howie Y. S. Huang L. Rozee D. F. Horrobin |
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Affiliation: | (1) Efamol Research Institute, P.O. Box 818, B4N 4H8 Kentville, Nova Scotia, Canada |
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Abstract: | Effects of supplementation of saturated fatty acids (16:0 and 18:0) on metabolism of the cytotoxic n-6 fatty acids in cultured human monocyte-like cells (U937) have been examined. U937 cells were incubated in 5% delipidated fetal bovine serum containing 16:0 and 18:0. Supplementation of either 16:0 or 18:0 has no significant effect on the uptake of 18:2n-6 and 18:3n-6. However, addition of 16:0 to the medium increased whereas 18:0 suppressed the cytotoxic effects of 18:2n-6 and 18:3n-6. In addition, 16:0 supplementation reduced the incorporation of n-6 fatty acids in cellular phospholipid fraction, and enhanced the metabolism of n-6 fatty acids, particularly the conversion of 20:3n-6 to 20: 4n-6 in U937 cells. Results with microsomes prepared from U937 cells also showed that 16:0 supplementation increased the 5 desaturase activity. This may be related in part to an increase in the availability of 20:3n-6, since results obtained in a separate study have shown that 16:0 competed with 20:3n-6 for incorporaton into the phospholipid molecule at sn-2 position. Increasing the availability and formation of long chain n-6 fatty acids, which are cytotoxic, might also be responsible for increasing cytotoxicity of 16:0 supplementation. |
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Keywords: | n-6 fatty acids /content/gk40501780h58h40/xxlarge916.gif" alt=" Delta" align=" BASELINE" BORDER=" 0" >5 desaturation incorporation peroxides cytotoxicity |
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