An Improved Method for Rapid Fixation and Embedding of Pteridophyte Plant Materials |
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Authors: | Santha Devi V K Lall Poonam Carg Anoop Saxena Madhulika Tiwari |
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Institution: |
a Electron Microscopy Section, National Botanical Research Institute, Lucknow, India |
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Abstract: | A rapid method for fixation and embedding of plant materials, especially pterido-phytes, is suggested. Addition of tannic acid following osmication improved the visualization of membranes. Staining en bloc with uranyl acetate between osmication and tannic acid is suggested for tissues infected with fungi and bacteria. |
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Keywords: | rapid fixation and embedding fungi bacteria pteridophytes tannic acid |
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