The Saccharomyces cerevisiae protein Ccz1p interacts with components of the endosomal fusion machinery |
| |
Authors: | Róa Kucharczyk Marta Hoffman-Sommer Iga Piekarska Gabriele Fischer von Mollard & Joanna Rytka |
| |
Institution: | Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland;and;Biochemie III, Fakultät für Chemie, Universität Bielefeld, Bielefeld, Germany |
| |
Abstract: | The yeast protein Ccz1p is necessary for vacuolar protein trafficking and biogenesis. In a complex with Mon1p, it mediates fusion of transport intermediates with the vacuole membrane by activating the small GTPase Ypt7p. Additionally, genetic data suggest a role of Ccz1p in earlier transport steps, in the Golgi. In a search for further proteins interacting with Ccz1p, we identified the endosomal soluble N -ethylmaleimide-sensitive factor attachment protein receptor Pep12p as an interaction partner of Ccz1p. Combining the ccz1 Δ mutation with deletions of PEP12 or other genes encoding components of the endosomal fusion machinery, VPS21, VPS9 or VPS45 , results in synthetic growth phenotypes. The genes MON1 and YPT7 also interact genetically with PEP12 . These results suggest that the Ccz1p–Mon1p–Ypt7p complex is involved in fusion of transport vesicles to multiple target membranes in yeast cells. |
| |
Keywords: | Ccz1p yeast late endosome vesicular transport |
|
|