氯化钾或谷氨酸对人神经母细胞瘤SH-SY5Y细胞钙离子通透性的影响

用人神经母细胞瘤SH-SY5Y细胞系作为研究对象，通过测定细胞存活率(MTT法)和脂质过氧化代谢产物丙二醛(MDA)，探讨了氯化钾(KCl)或谷氨酸分别对入神经母细胞瘤SH-SY5Y细胞的损伤作用。结果发现在含Ca

INFLUENCE OF POTASSIUM CHLORIDE OR GLUTAMATE ONCALCIUM ION PERMEABILITY OF HUMAN NEUROBLASTOMA SH-SY5Y CELL LINE

The present study investigated the injury of cultured human neuroblastoma SH-SY5Y cell line induced by potassium chloride(KCl) or glutamate, using the methods of MTT and TBA assays which measured the cell viability and malondialde-hyde (MDA), respectively. The results showed that, when the cultured SH-SY5Y cells were incubated with KCl(40 -80mmol/L)or glutamate (50 - 100mmol/L)for 4 hours in Hank's buffer containing Ca2 , the cell viability was significantly decreased and the MDA content standing for the degree of lipid percoddation increased; but the incubation of KC1 or glutamate with SH-SY5Y cells in Hank's buffer without Ca2 did not induce the obvious changes in cell viability and lipid peroxidation. The morphological changes observed through the phase-contrast microscope were in accord with above results. The experiments indicate that the cultured human neuroblastoma SH-SY5Y cell line can be injured by KQ or glutamate.and their injur effects are related to the inflow of extracellular Ca2 and lipid peroidation. This cell model may provide a quick and simple means for screening the neuro-protective drugs.