Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation |
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Authors: | Thomas Proft |
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Institution: | (1) Department of Molecular Medicine & Pathology, School of Medical Sciences, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand;(2) Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand |
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Abstract: | Sortases are transpeptidases produced by Gram-positive bacteria to anchor cell surface proteins covalently to the cell wall.
The Staphylococcus aureus sortase A (SrtA) cleaves a short C-terminal recognition motif (LPXTG) on the target protein followed by the formation of an amide bond with the pentaglycine
cross-bridge in the cell wall. Over recent years, several researchers have exploited this specific reaction for a range of
biotechnology applications, including the incorporation of non-native peptides and non-peptidic molecules into proteins, the
generation of nucleic acid–peptide conjugates and neoglycoconjugates, protein circularisation, and labelling of cell surface
proteins on living cells. |
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Keywords: | |
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