Biologically active human GM-CSF produced in the seeds of transgenic rice plants |
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Authors: | Ravinder Sardana Anil K Dudani Eilleen Tackaberry Zaman Alli Suzanne Porter Karen Rowlandson Peter Ganz Illimar Altosaar |
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Institution: | (1) Department of Biochemistry, Microbiology & Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON, Canada, K1H 8M5;(2) Present address: CIPO, Industry Canada, 50 Victoria Street, Gatineau, QC, Canada, K1A 0C9;(3) Biologics and Genetic Therapies Directorate, Health Canada, Tunney’s Pasture, Ottawa, ON, Canada, K1A 0L2;(4) Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON, Canada, K1H 8M5 |
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Abstract: | Rice flour is a well-known and characterized source of pharmaceutical ingredients, which are gluten-free and incorporated
in many drug delivery applications such as excipient starch. To further exploit this uniqueness, the synthetic capacity of
rice endosperm tissue, the basis of rice flour, was extended by genetic transformation. Recombinant human GM-CSF, a cytokine
used in treating neutropenia and with other potential clinical applications, has been expressed in transgenic rice seeds using
a rice glutelin promoter. Rice seeds accumulated human GM-CSF to a level of 1.3% of total soluble protein. The rice seed-produced
human GM-CSF was found to be biologically active when tested using a human cell line TF-1. Use of rice as a host plant offers
not only attractive features of safe production in seeds but also self-containment of foreign genes, as rice is primarily
a self-pollinated crop plant.
Ravinder Sardana and Anil K. Dudani contributed equally to this work. |
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Keywords: | GM-CSF Rice Transgenic Endosperm TF-1 cells |
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