Influence of Brassinosteroids on Antioxidant Enzymes Activity in Tomato Under Different Temperatures |
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| Authors: | L.M. Mazorra M. Núñez M. Hechavarria F. Coll M.J. Sánchez-Blanco |
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| Affiliation: | (1) Dpto. Fisiologia y Bioquímica Vegetal, Instituto Nacional de Ciencias Agrícolas (INCA), San José de Las Lajas, Gaveta Postal No.1 La Habana, Cuba;(2) Laboratorio de Productos Naturales, Facultad de Química, Universidad de La Habana, Zapata y G. Vedado C., La Habana, Cuba;(3) Dpto. Riego y Salinidad, Centro de Edafología y Biología Aplicada del Segura (CSIC), P.O.Box 4195, E-30080 Murcia, Spain |
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| Abstract: | The effect of brassinosteroids (BRs) on catalase (EC 1.11.1.6), peroxidase (EC 1.11.1.7) and superoxide dismutase (SOD, EC 1.15.1.1) activity in tomato leaf discs was analyzed at 25 and 40 °C. Tomato leaf discs were preincubated for 24 h in Petri dishes with 24-epibrassinolide (EBR) or a polyhydroxylated spirostanic analogue of brassinosteroids (MH5). Both concentrations (10.60 and 2.12 nM) of EBR and MH5 stimulated the activity of SOD at 25 and 40 °C, the MH5-stimulated increase of this enzyme activity was greater. Peroxidase activity was unaffected at 25 °C, while at 40 °C this activity was enhanced by both compounds. The changes in catalase activity markedly depended on the structure BRs, doses and temperature. The results suggest a possible role of EBR and MH5 in the reduction of cell damage produced by heat stress due to induction of enzymatic antioxidants. |
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| Keywords: | brassinosteroid analogue catalase heat stress Lycopersicon esculentum peroxidase superoxide dismutase |
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