Plasmodium falciparum: continuous cultivation of erythrocyte stages in plasma-free culture medium |
| |
Authors: | G P Willet C J Canfield |
| |
Institution: | Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Washington, D.C. 20307, U.S.A. |
| |
Abstract: | Continuous in vitro cultivation of the malaria parasite, Plasmodium falciparum, was performed in plasma-free medium. The medium used was standard RPMI 1640 supplemented with adenosine, unsaturated C-18 fatty acids, and fatty acid-free bovine serum albumin. The medium was changed daily and the cultures were diluted with washed erythrocytes twice weekly. Growth was routinely maintained for 1 month at which time the experiments were usually terminated. Although the overall growth rates were consistently lower than in control cultures with plasma, continuous growth occurred in the absence of plasma in cultures containing cis-vaccenic, oleic, and linoleic acids. |
| |
Keywords: | Protozoa parasitic Malaria human Medium defined Lipids Fatty acids |
本文献已被 ScienceDirect 等数据库收录! |
|