鰤鱼诺卡氏菌对乌鳢血液指标的影响

应用浓度为106cfu/mL的鰤鱼诺卡氏菌(Nocardia seriolea)菌悬液腹腔注射乌鳢,人工感染诺卡氏菌病。在感染后3d、6d、9d、12d、18d和24d时抽取乌鳢血液,检测乌鳢的血细胞数、血细胞脆性、溶菌酶、血清总蛋白、碱性磷酸酶、乳酸脱氢酶和尿素氮等指标。结果表明,与对照组相比,感染鰤鱼诺卡氏菌后乌鳢血细胞数呈先升高后降低的趋势,而血细胞脆性在各实验时间点均高于对照组;血清碱性磷酸酶呈先降低后升高趋势,血清溶菌酶、血清总蛋白和血清尿素氮等均呈先降低后增高再降低的趋势,血清乳酸脱氢酶活力显著增加。说明鰤鱼诺卡氏菌感染会引起乌鳢血液指标明显变化。

Effect of Nocardia seriolea on Blood Indices of Channa argus

This study is to eva luate the influences of Nocardia seriolae on the haematological parameters of Channa argus. C. argus were injected intraperitoneally with 10 6 cfu/mL N. seriolae suspension. Blond samples were taken at 3 d, 6 d, 9 d, 12 d, 18 d and 24 d after that injection,then the number of erythrocyte, blood cell fragility, lysozyme, serum total protein, alkaline phosphatase, lactic dehydrogenase, urea nitrogen were tested. The results showed that compared with control group, the number of erythrocyte increased at first and then decreased, while blood cell fragility was higher than control group all the time. Alkaline phosphatase decreased at first and then increased. Lysozyme, serum total protein and urea nitrogen had the same tendency change that they all decreased at first and then increased and decreased again. The activity of lactic dehydrogenase increased significantly. These indicate that tissues of Channa argus, such as liver, kidney, heart and spleen, can be damaged seriously and the blood indices may change significantly when it is infected with N. seriolea.