A genetic linkage map of the MSM Japanese wild mouse strain with restriction landmark genomic scanning (RLGS) |
| |
Authors: | Shingo Akiyoshi Hiroaki Kanda Yasushi Okazaki Tomoya Akama Kimie Nomura Yoshihide Hayashizaki Tomoyuki Kitagawa |
| |
Institution: | (1) Department of Pathology, the JFCR Cancer Institute, 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170-8455, Japan, JP;(2) Genome Science Laboratory, RIKEN Tukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyodai, Tsukuba City, Ibaraki 305-0074, Japan, JP |
| |
Abstract: | A high-resolution genetic map of the Mus musculus molossinus (MSM) Japanese wild mouse strain was constructed with restriction landmark genomic scanning (RLGS) and compared with that
of the laboratory strain C3H. MSM is phylogenetically 1 million years apart from common laboratory mouse strains and is distinctly
resistant to chemical carcinogenesis. Since it exhibits frequent genetic polymorphisms with laboratory mice but can still
be easily crossed with laboratory strains, hybrids between MSM and carcinogen-sensitive laboratory mouse strains provide excellent
materials for analysis of modifier genes and genetic changes during carcinogenesis. We have generated MSM backcross progeny
with the C3H strain, which is extremely sensitive to hepatocarcinogenesis, to construct the present map. RLGS profiles with
two combinations of restriction enzymes (NotI–PvuII–PstI, NotI–PstI–PvuII) yielded more than 2000 spots each. The polymorphism rate was about 39.2%, and of a total of 1732 polymorphic spot loci
identified, 1371 could be assigned to specific chromosomes by comparison with 79 microsatellite marker loci. Thus, 1450 loci,
on all chromosomes except for Y, effectively mapped 90% of the genome (1431.7 cM length). Although some spots might be derived
from the same NotI site, each NotI site potentially generating two fragments, the presence of at least 515 loci groups with different progeny distribution
patterns dispersed through the genome with an average spacing of 3 cM, means that this genetic map should be useful for analysis
of various biological phenomena, including carcinogenesis and ontogenesis, at the gene level.
Received: 25 August 1999 / Accepted: 20 December 1999 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|